Research progress of enzyme-instructed self-assembly molecules for tumor therapy and imaging / 中国药科大学学报

@#Self-assembly is the basis of the formation of biological macromolecular structure. Enzyme-instructed self-assembly (EISA) with the help of tool enzymes, realizing the conversion of small molecular compounds to supramolecular nanostructures at specific sites, become a new strategy for drug discovery.In recent years, the exploration of EISA for developing malignant cancer therapy and imaging has made considerable progress, achieving the precise regulation and tumor targeting of nanostructures. This paper reviews the latest progress of EISA in the field of tumor diagnosis and treatment, the functions and characteristics of tool enzymes such as alkaline phosphatase, sirtuin, tyrosinase, γ-glutamyltranspeptidase and caspase-3,summarizes the research status of EISA targeting multiple organelles in tumor therapy, and introduces the application of EISA in tumor imaging, aiming to provide reference forthe research of EISA strategy in tumor diagnosis and treatment.

Professor LI Qiyi's Clinical Experience in Diagnosing and Treating Insomnia from Phlegm and Blood Stasis / 浙江中医药大学学报

[Objective] To summarize Professor LI Qiyi's clinical experience in diagnosing and treating insomnia from phlegm and blood stasis, who is the national traditional Chinese medicine experts academic experience inheritance work guidance teacher.[Method] To study and research Professor LI Qiyi's papers and books, to follow his guidance, to collect and arrange typical cases record, to summarize Professor LI Qiyi's methods and empirical medication in diagnosing and treating insomnia from phlegm and blood stasis.In order to analyse her experience, a clinical case was reported.[Result]Professor LI Qiyi treats the insomnia from phlegm and stasis, based on promoting blood circulation for removing blood stasis and reducing phlegm.Some people mixed with liver stagnation, are treated with clearing and calming heart, soothing liver and relieving depression.Some of others mixed with phlegm heat disturbance heart, are treated with clearing away heat and resolving phlegm, calming heart and tranquilizing mind.Because of being combined with symptoms and syndromes of TCM syndrome differentiation and treatment, satisfied results are predicted, the case shows the experience of TCM treating insomnia.[Conclusion]Professor LI Qiyi's experience in treating insomnia from phlegm and blood stasis is well worth learning for its significant clinical curative effect.

Litterature review TCM treatment for insomnia / 国际中医中药杂志

Traditional Chinese medicine (TCM) treatment for insomnia focused on the balance of Yin and Yang of internal organs. As to the treatment, Chinese medicine has a variety of treatment methods, such as herbal medicine, acupuncture, moxibustion, Guasha and so on. Besides, TCM also emphasized the importance to the comprehensive adjustment of life style and mental condition. This article summarized the clinical treatment forinsomnia from manyaspects, such as treatment according to the TCM patterns differentiationof internal organs, herbal medicine, acupuncture, life-style adjustment and other common therapies.

Professor Li Qiyi's Experience in Treating Coronary Heart Disease / 浙江中医药大学学报

[Objective] The article summarized and discussed professor Li Qiyi's experience in treating coronary heart disease. [Methods] Discuss the experience of professor Li Qiyi in treating coronary heart disease from cause of disease, pthogenesis, combination of syndrome diferentiation and disease and so on. [Results] The pathogenesis of coronary heart disease is“deficiency of both qi and Yin, blockage of phlegm and blood stasis”. Professor Li Qiyi advocated we should combinate both syndrome diferentiation and disease according to the cause of disease and pthogenesis. Professor Li Qiyi often uses“Guanxinping”as basic decoction treatment in clinic and it can have excellent cure effect. [Conclusion] Professor Li Qiyi takes both Tongbi tonic and application of comprehensive therapy to tonify“qi and Yin”and eliminate phlegm and blood stasis which can have good cure effect . This method can relieve the clinical symptoms of the patients of coronary heart disease and delay the progress of the disease and then the patients can live a better life.

Complications and prophylaxis strategies of posterior hemivertebra resection for congenital scoliosis / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the surgical outcomes and related complications of posterior hemivertebra resection with transpedicular instrumentation in the treatment of congenital scoliosis caused by fully-segmented non-incarcerated hemivertebra.</p><p><b>METHODS</b>From January 2003 to January 2012, one hundred and forty consecutive cases of congenital scoliosis treated by posterior hemivertebra resection with transpedicular instrumentation were investigated retrospectively. Radiographs were reviewed to determine the type and location of the hemivertebra, the coronal curve magnitude, sagittal alignment, compensatory cranial curve and compensatory caudal curve preoperatively, postoperatively and at the latest follow-up. Operative reports and patient charts were reviewed to record operation time, fusion level and complications.</p><p><b>RESULTS</b>One hundred and fifty-one posterior hemivertebra resections in 140 patients aged 2 to 45 years (average 10.8 years) with non-incarcerated hemivertebra were evaluated. All the patients were followed up from 3 to 119 months (average 25 months). The average fusion level was 5.0 segments (2-11 segments). There was a mean improvement of 71.3% in the segmental scoliosis from 42.5° before surgery to 10.6° at the time of the latest follow-up, and a mean improvement of 66.8% in segmental kyphosis from 29.5° to 7.2° at the same periods. There were 14 complications (13 patients), 3 pedicle fractures, 2 rod breakages, 2 pedicle elongation, 2 removed implants for prominent implants, 2 delayed wound healing, 2 additional surgeries for curve progression, 1 prolonged respiratory support. There was no neurological complication.</p><p><b>CONCLUSIONS</b>Posterior hemivertebra resection with transpedicular instrumentation is a safe and effective procedure for congenital scoliosis patients.Neurological complication is rare, but implant-related complication still remains a challenge.</p>

Effects of collagen matrices by covalent incorporation of heparin and loading with huangqi injection (HI) on anagenetic blood vessel / 中国中药杂志

To investigate effects of collagen matrices by covalent incorporation of heparin and loading with huangqi injection (HI) on anagenetic capillaries, we established the chick chorioallantois model. The collagen matrices by covalent incorporation of heparin and loading with HI were placed and then the eggs were continuously incubated for 3 days. The number of capillaries in the vicinity of samples, the hemoglobin content inside the samples, the dry weight and the macroscopic observation were evaluated. We found the heparinized matrices had comparable angiogenic effects. The number of capillaries, the hemoglobin content, the expression of CD34 increased remarkably (P < 0.01). So we concluded that HI might be considered as an alternative or addition agent to promote the acidification of capillaries.

Surgical procedures for chronic pancreatitis associated with pancreatic duct stones / 中华普通外科杂志

Objective To investigate the surgical procedures for chronic pancreatitis associated with pancreatic duct stones.Methods The clinical data of 17 cases of chronic pancreatitis with pancreatic duct stones surgically treated were analyzed retrospectively.Results Of the 17 cases,13 had pancreatic duct stones in the head of the pancreas.4 had pancreaticolithiasis in the body and tail of the pancreas,six had additional choledocholithiasis.Surgical treatments included Roux-en-Y anastomosis of the pancreatic duct and jejunum in 6 cases(Partington procedure),anastomosis of pancreatic duct and stomach in 4 cases (Warren procedure).subtotal resection of the head of the pancreas with duodenal preservation in 3 cases (Beger's procedure).removal of the tail of pancreas with Roux-en-Y anastomosis of the distal pancreatic end to the iejunum in 3 cases(Duval's procedure),removal of the tail of pancreas and spleen with a Roux-en-Y anastomosis of the distal pancreatic end to ieiunum in 1 case.All 17 patients were cured,with complete relief of intractable abdominal pain in 15 cases.blood glucose was under eontrol in two out of six diabetics.Two cases suffered from postoperative pancreatic fistula.one patient died of pancreatic cancer 11 months after operation.Conclusion For patients with chronic pancreatitis and pancreaticolithiasis.surgical treatment should be highly individualized.We suggest drainage procedures for patients with dilatation of the pancreatic duct.Patients with no dilatation of the pancreatic duct and those with suspected carcinoma can be treated by partial pancreatectomy and Roux-en-Y pancreaticojejunostomy.Meanwhile effort must be applied to preserve the exocnne and endocrine pancreatic function vital for the patient's quality of life.

The value of three-dimensional CT reconstruction imaging in the diagnosis and treatment of congenital scoliosis / 中华骨科杂志

Objective To analyze the value of three-dimensional computed tomography (CT) reconstruction imaging for the diagnosis and treatment of congenital scoliosis. Methods 76 patients with congenital scoliosis were examined with X-ray of total spine and three-dimensional CT reconstruction, and the results were analyzed. Compared with X-ray film, the findings with the CT imaging were classified into three groups: Group Ⅰ, no supplementary useful information was obtained; Group Ⅱ, further confirmation of findings which were unclear or ambiguous on X-ray film, with improved visualization and comprehension of the type of the deformity; Group Ⅲ, substantial new information was obtained. Results Group Ⅰ: 11 (14.5%) patients, without supplementary useful information obtained with three-dimensional CT reconstruction imaging. The patients aged from 2 to 16 years(11.00?10.09 years), and the Cobb angle ranged from 28? to 102?(55.60??21.06?). Group Ⅱ: 49 (64.5%) patients, with confirmatory finding or improved visualization and comprehension of the previously identified deformities obtained with CT imaging. The 49 patients aged from 4 to 28 years (13.53?4.47 years), and the Cobb angle ranged from 20? to 115? (55.41??23.44?). Group Ⅲ: 16 (21%) patients, with substantial new information obtained with CT reconstruction images which was unrecognized with X-ray film. The patients aged from 6 to 16 years (12.88?2.68 years),and the Cobb angle ranged from 37? to 145?(73.19??28.18?). The difference of age of patients between 3 groups was of no significance (P=0.052), but the difference of Cobb angle between 3 groups was significant statistically (P=0.039). Conclusion Three-dimensional and multiplanar reformatted CT imaging allows better visualization and understanding of the deformities of scoliosis, and supplementary useful information may be obtained which was obscure or unrecognized with X-ray film, especially for patients with severe deformities. Its application in clinical practice may contribute to the diagnosis of the type of deformity and the segments involved, as well as the individualized operative planning.

The clinical manifestation and operative treatment for the scoliosis of Marfan's syndrome / 中华骨科杂志

Objective To evaluate the clinical manifestation and operative treatment for the scolio-sis of Marfan syndrome, and analyze its clinical outcomes as well. Methods The retrospective study cov-ered 10 patients including 8 females and 2 males with an average age of 12.9 years (9 to 16 years), who had undergone operations from January 1990 to December 2002. The Cobb's angle in the coronal and sagittal plane, the trunk shift, the apex rotation and shift were evaluated both before and after operation respectively. In the group, there were four cases with family history. Of the 10 cases, the classification of scoliosis was single curve in two cases, double curves in six cases and three curves in two cases. Results All the pa-tients were followed up for a mean time of 15 months, ranged from 4 months to 3 years. The trunk shift changed from the mean distance of 2.17 cm to 1.41 cm. The apex rotation decreased about 1?. The apex shift changed from the mean distance of 4.57 cm to 2.14 cm. The mean Cobb's angle in the coronal plane changed from 88? to 42?. The correction rate was about 46.38% (18.18% to 81.54%). The Cobb's angle on the final follow-up was 46?( 11? to 96?), and the mean lost angle was 4?. The mean Cobb's angle of the thoracic kyphosis was 20?(-10? to 52?) preoperatively and 23?(0? to 35?)postoperatively, and 24?( 0? to 35?) on the final follow-up. The Cobb's angles of the thoracolumbar kyphosis of 5 cases improved from 85? to 10? after operations, and there was no angle loss on the follow-up. Conclusion The three dimensional corrective instrumentation can be used effectively for the correction of the scoliosis of Marfan syndrome. The critical points for the successful operation are the simultaneous correction of the coronal and sagittal plane deformities, prevention of the dural expansion in the lumbar or lumbosacral canal, rigid and multi-segmental internal fixation, extensive and ample bone fusion, and correct external fixation after operation.